tgn46 tgoln2  (Bio-Rad)

Journal: Cellular & Molecular Biology Letters

Article Title: Golgi-associated retrograde protein (GARP) complex-dependent endosomes to trans Golgi network retrograde trafficking is controlled by Rab4b

doi: 10.1186/s11658-024-00574-w

Figure Lengend Snippet: List of antibodies and ligands

Article Snippet: TGN46/TGOLN2 , Rabbit polyclonal, AbD serotec #AHP1586 , IF/TEM.

Techniques: Binding Assay

Journal: Cellular & Molecular Biology Letters

Article Title: Golgi-associated retrograde protein (GARP) complex-dependent endosomes to trans Golgi network retrograde trafficking is controlled by Rab4b

doi: 10.1186/s11658-024-00574-w

Figure Lengend Snippet: Localization of Rab4b wt and S22N. A EEA1, CI-M6PR, TGN46, p230, or GM130 labeling (red) in HeLa cells expressing the same amount of GFP-Rab4b (wt) or GFP-Rab4b S22N (green) shown as merged images. were treated for immunofluorescence using antibodies and anti-species Texas Red-coupled secondary antibodies. The bar corresponds to 10 µm. Enlarged views of the demarcated area are also shown. Arrowheads indicate to GFP-Rab4b-positive vesicles containing M6PR, whereas arrows point to Rab4b-positive structures not containing M6PR. B merged images of GFP-GOLPH (green) and HA-tag (red) labeling. The bar corresponds to 10 µm. Enlarged views of the delineated area are shown. C , D TEM images of GFP-Rab4b (10 nm gold particles) and TGN46 C or CI-M6PR D (15 nm gold particles) using mouse anti-GFP and rabbit anti-TGN46 C or rabbit anti-GFP and mouse anti-CI-M6PR ( D ). Enlarged views of the delineated area are shown and bars are for 500 nm

Article Snippet: TGN46/TGOLN2 , Rabbit polyclonal, AbD serotec #AHP1586 , IF/TEM.

Techniques: Labeling, Expressing, Immunofluorescence

Journal: Autophagy

Article Title: Transiently expressed ATG16L1 inhibits autophagosome biogenesis and aberrantly targets RAB11-positive recycling endosomes

doi: 10.1080/15548627.2016.1256521

Figure Lengend Snippet: Identification of the aberrant targeted membrane by transiently expressed ATG16L1. A549 cells were infected with Ad-GFP-ATG16L1 (MOI 2) for 16 h. Cells were then fixed and probed with a cis-Golgi marker GOLGA2/GM130 (A), trans-Golgi marker TGOLN2/TGN46 (B), ER Golgi intermediate compartment marker (LMAN1/ERGIC-53) (C), lysosome marker LAMP2 (D), or late endosome marker RAB7 (E). Boxed areas are enlarged to show the association between GFP-ATG16L1+ puncta and corresponding subcellular compartments. Scale bar: 25 micron.

Article Snippet: Chemicals and antibodies Wortmannin (Cell Signaling Technology, 9951); bafilomycin A 1 (LC Laboratories, B1080); chloroquine diphosphate salt (Sigma-Aldrich, C6628); TGOLN2/TGN46 antibody (Sigma-Aldrich, T7576); Alexa Fluor 555-conjugated TRF (ThermoFisher Scientific, {"type":"entrez-nucleotide","attrs":{"text":"T35352","term_id":"617450","term_text":"T35352"}} T35352 ); fluorescein-conjugated human TRF (ThermoFisher Scientific, T2871).

Techniques: Infection, Marker